Project Director

Symes, Steven

Department Examiner

Mebane, Robert; Ledoan, Andrew; Kim, Jisook

Department

Dept. of Chemistry

Publisher

University of Tennessee at Chattanooga

Place of Publication

Chattanooga (Tenn.)

Abstract

The creation of an electrospun nanofiber embedded with two antibiotics that will be used to prevent infection while also stimulating bone regeneration will be explored. The nanofiber will contain an aminoglycoside and a glycopeptide, and it is sought to deliver a sustained release of antibiotics for up to 12 weeks. To determine release rate, nanofiber samples are immersed in 10.0 mL of MiliQ water (MQ), incubated at 37°C, assayed at specific time intervals, and then analyzed using ultra performance liquid chromatography in tandem with mass spectrometry. A hydrophilic interaction liquid chromatography (HILIC) method utilizing ultra-performance liquid chromatography (UPLC) in tandem with mass spectrometry (MS) has been developed in order to quantify the release rate of each antibiotic from the nanofiber mesh as a function of time spent in MQ. The antibiotic release rate for six separate permutations of nanofibers was documented. In addition to monitoring drug release as a function of time, mass loss, pH changes, and morphology changes as a function of time have been monitored for an additional three antibiotic-free permutations of nanofiber devices. More specifically, the mass loss profiles for the three permutations of nanofiber devices were generated. To determine mass loss, nanofiber samples are immersed in 10.0 mL of a phosphate buffered saline (PBS), incubated at 37°C, assayed at specific time intervals, and then a final mass was directly compared to the starting mass. Changes in morphology were documented using a scanning electron microscope (SEM). SEM images were taken at weekly intervals and compared to original SEM images characterizing the nanofibers before immersion in PBS.

Acknowledgments

Dr. Symes Patrick O'Brien Daniel Shults Marcus Morgan UTC Grote Fund PSRA UTCOM US Army UTC Department of Chemistry

Degree

B. S.; An honors thesis submitted to the faculty of the University of Tennessee at Chattanooga in partial fulfillment of the requirements of the degree of Bachelor of Science.

Date

12-2015

Subject

Bone regeneration; Biomedical materials; Nanostructured materials

Keyword

limb salvage device; electrospun nanofiber device; nanofiber; antibiotic release

Discipline

Chemistry

Document Type

Theses

Extent

43 leaves

Language

English

Rights

Under copyright.

License

http://creativecommons.org/licenses/by-nc-nd/3.0/

Date Available

12-31-2018

Available for download on Monday, December 31, 2018

Included in

Chemistry Commons

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