Harris, Bradley J.
University of Tennessee at Chattanooga
Place of Publication
Vibrio cholerae, a Gram-negative bacterium, is responsible for the acute intestinal infection known as cholera. This illness is due in part to V. cholerae’s ability to sense and adapt to changing environments as it is ingested into the human body from brackish environments. It was shown in recent studies that this bacteria has the ability to uptake exogenous fatty acids, resulting in changes to V. cholerae persistence and pathogenicity. The aim of this research is to determine the extent to which these additional exogenous UFAs influence the persistence and pathogenicity of V. cholerae throughout its transitional period from brackish environments to human host conditions. Our hypothesis is that environmental conditions (e.g., temperature, pH, salinity) direct fatty acid-induced changes in V. cholerae’s capacity to spread disease by triggering changes in the bacterium’s virulence factors such as: biofilm formation, antibiotic resistance, and cholera toxin production. Initial work focused on identifying the environmental conditions that trigger fatty acid-induced changes in V. cholerae persistence and pathogenicity using a design of experiments approach. The final work of this project focused on literary analysis of effect of the exogenous fatty acids on V. cholerae’s genetic regulatory pathways.
Dr. Bradley Harris, Ph.D Dr. David Giles, Ph.D
B. S.; An honors thesis submitted to the faculty of the University of Tennessee at Chattanooga in partial fulfillment of the requirements of the degree of Bachelor of Science.
Vibrio cholerae; Microbial toxins -- Analysis
Doyle, Abigail, "Influence of environmental conditions on fatty acid-induced changes in Vibrio cholerae persistence and pathogenicity" (2019). Honors Theses.