Harris, Bradley J.
Giles, David; Danquah, Michael
University of Tennessee at Chattanooga
Place of Publication
Antibiotic-resistant pathogens represent an escalating threat to public health worldwide, substantially increasing the burden of healthcare and community-acquired infections. Several factors contribute to the emergence and spread of this threat, including but not limited to improper antibiotic use and prescriptions in health-care settings and the community, increasing global travel and migration from countries that have higher levels of antibiotic-resistant pathogens, and a lack of new antibiotics under development. According to the World Health Organization, rising rates of resistance among Gram-negative bacteria (such as Vibrio cholerae) are of particular concern. These bacteria have evolved a number of endogenous membrane remodeling strategies to sense and adapt to their environment. However, another membrane remodeling strategy employed by these bacteria, the uptake and assimilation of exogenous long- chain fatty acids, remains largely unexplored. Here, we seek to address this knowledge gap by determining the extent to which phospholipid remodeling through uptake of exogenous PUFAs impacts antimicrobial resistance in V. cholerae. As expected, resistance to polymyxin B is substantially lower for mutants lacking the ability to modify LPS compared to the wild-type (El Tor) strain. More interestingly, PMB resistance varies when cells are grown in the presence of long-chain fatty acids. This difference is most noticeable for long-chain fatty acids abundant in the human intestines.
Dr. Bradley Harris, Ph.D Dr. David Giles, Ph.D, Konner Glass, Devin Martin
B. S.; An honors thesis submitted to the faculty of the University of Tennessee at Chattanooga in partial fulfillment of the requirements of the degree of Bachelor of Science.
Antibiotics; Phospholibids; Vibrio cholerae
Bacteriology | Pharmacology
v, 25 leaves
Strike, William, "Phospholipid remodeling via exogenous polyunsaturated fatty acid uptake modulates stress resistance in Vibrio cholerae" (2020). Honors Theses.