Craddock, J. Hill
Shaw, Joey; Barbosa, Jose
College of Arts and Sciences
University of Tennessee at Chattanooga
Place of Publication
Chestnut breeders routinely screen hybrid chestnut seedling progeny for resistance to chestnut blight in an orchard setting – a process that takes five to seven years. A novel technique that allows for screening in the first and second growing season is known as a small stem assay. We screened 110 first-backcross seedlings and 98 third-backcross seedlings in a completely randomized design in 2017 (Gentner 2018). In 2018, an additional 391 F2 hybrid chestnut seedlings were screened in a randomized complete block design. All seedlings were inoculated with the chestnut blight causal organism Cryphonectria parasitica when stem diameters were greater than 4mm. The 2017 trial recorded the day on which the plant wilted, during both the 2017 and 2018 trial, canker length was measured and recorded. The 2017 trial gave insight to the rate at which different seed types wilted and the 2018 trial selected the very best of the F2s.
M. S.; A thesis submitted to the faculty of the University of Tennessee at Chattanooga in partial fulfillment of the requirements of the degree of Master of Science.
American chestnut; Chinese chestnut; Plant bioassay; Trees -- Breeding
xiii, 54 leaves.
Miller, Margaret Joyce, "Small stem assay for chestnut blight resistance in segregating half-sib and full-sib families of F2 and backcross hybrid chestnut trees" (2020). Masters Theses and Doctoral Dissertations.