Project Director
Kim, Jisook
Department Examiner
Albu, Titus; Whitson, Stephanie; Nichols, Stephen
Publisher
University of Tennessee at Chattanooga
Place of Publication
Chattanooga (Tenn.)
Abstract
Protein modifications may occur upon exposure to environmental toxins such as polycyclic aromatic hydrocarbon (PAH) molecules or their metabolites. In this context, our laboratory was interested in investigating protein modifications in the presence of select naphthoquinones, which were 2-hydroxy-1,4-naphthoquinone (HNQ) and 1,2-naphthoquinone (o-NQ). The effects of HNQ and o-NQ on the protein Ribonuclease A (RNase) were investigated through a variety of conditions. These modified incubation conditions included pH variation and the addition of metal ions to further mimic physiological conditions. Documentation of results was carried out through sodium dodecyl sulfate polyacrylamide gel electrophoretic analysis (SDS-PAGE). Of the two quinones, o-NQ exhibited a greater level of activity toward RNase. HNQ was found to be stable under the studied conditions, resulting in almost no observed RNase modification. Linking environmentally abundant compounds to toxicological effects on biologically significant molecules can help to set precedents toward their usage and disposal that have a positive ripple effect.
Acknowledgments
Dr. Jisook Kim, Dr. Titus Albu, Dr. Stefanie Whitson, Dr. Stephen Nichols, The UT Chattanooga Chemistry Department, The Grote Chemistry Fund, and the Provost Student Research Award.
Degree
B. S.; An honors thesis submitted to the faculty of the University of Tennessee at Chattanooga in partial fulfillment of the requirements of the degree of Bachelor of Science.
Date
5-2015
Subject
Proteins -- Analysis
Discipline
Chemistry
Document Type
Theses
Extent
64 leaves
DCMI Type
Text
Language
English
Rights
http://rightsstatements.org/vocab/InC/1.0/
License
http://creativecommons.org/licenses/by-nc-nd/3.0/
Date Available
12-30-2018
Recommended Citation
Smith, Michelle Dawn, "Ribonuclease A modification induced by 1,2-Naphthoquinone and 2-Hydroxy-1,4-Naphthoquinone" (2015). Honors Theses.
https://scholar.utc.edu/honors-theses/34
Department
Dept. of Chemistry