Project Director

Harris, Bradley

Department Examiner

Yang, YingFeng; Danquah, Michael

Department

Dept. of Civil and Chemical Engineering

Publisher

University of Tennessee at Chattanooga

Place of Publication

Chattanooga (Tenn.)

Abstract

This study explores the bioaffinity of aptamer-target interactions through a comprehensive analysis. Aptamers, oligonucleotides that can bind to target molecules, have emerged as powerful tools in various fields, including diagnostics and therapeutics. The primary objective of this research was to assess the binding affinity and specificity of selected aptamers to their respective targets using a combination of experimental techniques and computational models. The binding affinity of 10 different complexes were measured through surface plasmon resonance (SPR) followed by molecular simulations. The results showed that the 6th complex had the best binding affinity. The data suggest that these aptamers could be valuable for developing sensitive biosensors and targeted therapeutic agents.

Acknowledgments

Thank you to Dr. Bradley Harris and Dr. Michael Danquah

Degree

B. S.; An honors thesis submitted to the faculty of the University of Tennessee at Chattanooga in partial fulfillment of the requirements of the degree of Bachelor of Science.

Date

8-2024

Subject

Binding sites (Biochemistry); Oligonucleotides; Protein Binding; Nucleotide sequence--Methodology

Keyword

Bioaffinity; Molecular simulations; Aptamer; COVID-19

Discipline

Molecular, Cellular, and Tissue Engineering

Document Type

Theses

Extent

i, 32 leaves

DCMI Type

Text

Language

English

Rights

http://rightsstatements.org/vocab/InC/1.0/

License

http://creativecommons.org/licenses/by/4.0/

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