Project Director
Harris, Bradley
Department Examiner
Yang, YingFeng; Danquah, Michael
Publisher
University of Tennessee at Chattanooga
Place of Publication
Chattanooga (Tenn.)
Abstract
This study explores the bioaffinity of aptamer-target interactions through a comprehensive analysis. Aptamers, oligonucleotides that can bind to target molecules, have emerged as powerful tools in various fields, including diagnostics and therapeutics. The primary objective of this research was to assess the binding affinity and specificity of selected aptamers to their respective targets using a combination of experimental techniques and computational models. The binding affinity of 10 different complexes were measured through surface plasmon resonance (SPR) followed by molecular simulations. The results showed that the 6th complex had the best binding affinity. The data suggest that these aptamers could be valuable for developing sensitive biosensors and targeted therapeutic agents.
Acknowledgments
Thank you to Dr. Bradley Harris and Dr. Michael Danquah
Degree
B. S.; An honors thesis submitted to the faculty of the University of Tennessee at Chattanooga in partial fulfillment of the requirements of the degree of Bachelor of Science.
Date
8-2024
Subject
Binding sites (Biochemistry); Oligonucleotides; Protein Binding; Nucleotide sequence--Methodology
Discipline
Molecular, Cellular, and Tissue Engineering
Document Type
Theses
Extent
i, 32 leaves
DCMI Type
Text
Language
English
Rights
http://rightsstatements.org/vocab/InC/1.0/
License
http://creativecommons.org/licenses/by/4.0/
Recommended Citation
Karunanithi, Pooja, "Bioaffinity analysis of aptamer-target interactions" (2024). Honors Theses.
https://scholar.utc.edu/honors-theses/588
Department
Dept. of Civil and Chemical Engineering