Committee Chair

Danquah, Michael

Committee Member

Mahtabi Oghani, Mohammad Javad, 1982-; Harris, Bradley; Wu, Dalei

Department

Dept. of Computational Science

College

College of Engineering and Computer Science

Publisher

University of Tennessee at Chattanooga

Place of Publication

Chattanooga (Tenn.)

Abstract

Aptamers are short polynucleotide chains that can bind with very high affinities to a broad range of chemical and biomolecular targets, including proteins, cells, viruses, microorganisms, toxins, and chemical compounds. They are analogous to antibodies with applications in therapeutic delivery, biosensors, and diagnostics, but possess the advantages of easy production and lower immunogenicity than antibodies. They are produced by the experimental method known as the systematic evolution of ligands by exponential enrichment (SELEX). SELEX is a powerful experimental method for selecting oligonucleotide aptamers for chemical or biomolecular targets of interest. The SELEX protocol has seen several modifications since its development in 1990, with the primary goal of improving its efficiency and speed. Recent advances to the original SELEX protocol, such as negative SELEX, capillary electrophoresis SELEX, and cell SELEX have increased the efficacy, speed, and target diversity of the original protocol significantly. However, regardless of these advances in the protocol, significant challenges continue to exist, impacting its widespread application for rapid generation of aptamers. Amongst the challenges, reagent consumption, efficiency, and time demand are the major issues for which the present study addressed through in-silico approaches. The developed in-silico approach can be used to augment experimental SELEX protocols for rapid screening of aptamer candidates, providing an integrated method that can speed up the selection process as well as biophysical characterization of aptamer candidates in the discovery process. Concepts and algorithms from machine learning, molecular docking, RNA structure prediction, conventional/enhanced molecular dynamics simulations, and free energy calculations made it possible to develop the in-silico workflow. The work reported in this thesis covers the computational tools, algorithms, and methods developed and implemented to select high-affinity aptamers for SARS-CoV-2 nucleocapsid and Staphylococcus aureus IsdA proteins with excellent binding properties based on small- molecule Fluorescence Resonance Energy Transfer (FRET) measurements.

Degree

Ph. D.; A dissertation submitted to the faculty of the University of Tennessee at Chattanooga in partial fulfillment of the requirements of the degree of Doctor of Philosophy.

Date

8-2023

Subject

Oligonucleotides; Coronaviruses; Staphylococcus aureus; Proteins

Keyword

aptamer, SELEX,FRET,IsdA,protein

Document Type

Doctoral dissertations

DCMI Type

Text

Extent

xii, 76 leaves

Language

English

Rights

http://rightsstatements.org/vocab/InC/1.0/

License

http://creativecommons.org/licenses/by/4.0/

Date Available

5-1-2024

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